Yes. The Touch 'n Go Business Card can be used for transit fare payment on supported participating rail and transit services where Touch 'n Go cards are accepted, including LRT, MRT, Monorail, BRT and other participating transit services.
However, upon launch, the Touch 'n Go Business Card is not accepted and cannot be used for transit fare payment including My50 on the MRT Kajang Line. Acceptance of the Business Card on the MRT Kajang Line may be introduced in the future update.